We then designed a small-molecule inhibitor that efficiently blocks HtrA activity, E-cadherin cleavage and intercellular entry of H. pylori designed to access the intercellular space. The unique electronic circuit of the EVOM2 and the included STX2 elec-trode detect the confluence of the cellular monolayer. The EVOM2 not only qualitatively measures cell monolayer health, but also quantitatively measures cellular confluence. E-cadherin shedding disrupts epithelial barrier functions allowing H. EVOM2 is the next generation, redesigned for ease of use. Here, we describe an entirely new function of HtrA and identify it as a new secreted virulence factor from Helicobacter pylori, which cleaves the ectodomain of the cell-adhesion protein E-cadherin. Mammalian and prokaryotic high-temperature requirement A (HtrA) proteins are chaperones and serine proteases with important roles in protein quality control. The Millicell-ERS (Electrical Resistance System) reliably measures membrane potential & resistance of epithelial cells in culture. ![]() Measurement of Transepithelial Electrical Resistance (TEER), Cell Monolayer Health Assessment, Quantitative Measurement of Confluence EVOM was the first instrument designed to perform routine Trans Epithelial Electrical Resistance (TEER) measurement in tissue culture research. Widely cited and recommended in epithelial cell culture protocols Measurements do not compromise sterility or damage cells on the membrane Portable instrument that fits into laminar flow hoods Accessories include a replacement (fixed) electrode set, adjustable electrode set and a specialized electrode set for use with Millicell®-96 cell culture insert plates. Due to the small size of the electrodes, you can easily measure transepithelial voltage and the resistance of cells grown on microporous membranes. ![]() Sarasota, City, FL), equipped with a pair of STX-2 chopstick. EVOM2 is the next generation, redesigned for ease of use. A silver/silver chloride (Ag/AgCl) pellet on each electrode tip measures voltage. Epithelial Tissue Voltohmmeter (World Precision Instruments. The EVOM was the first instrument designed specifically to perform routine Trans Epithelial Electrical Resistance (TEER) measurement in tissue culture research. This device qualitatively measures cell monolayer health and quantitatively measures cell confluence. The Millicell® ERS-2 (Electrical Resistance System) reliably measures membrane potential and resistance of epithelial cells in culture.
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